Device

Part:BBa_K189104

Designed by: GuoQiang Chen   Group: iGEM09_Tsinghua   (2009-10-20)

Therapeutic DNA

Therapeutic DNA containing a cos site, Amp resistance gene and O, P genes for replication.

We have characterized the co-functioning of this part with our designed GenSniper genome. However, whether a stuffer is needed for the package of Therapeutic DNA into GenSniper viroin should be further identified.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 53
    Illegal EcoRI site found at 4411
    Illegal SpeI site found at 3236
    Illegal PstI site found at 1547
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 53
    Illegal EcoRI site found at 4411
    Illegal NheI site found at 1388
    Illegal SpeI site found at 3236
    Illegal PstI site found at 1547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 53
    Illegal EcoRI site found at 4411
    Illegal BglII site found at 3997
    Illegal BglII site found at 4057
    Illegal BamHI site found at 5734
    Illegal XhoI site found at 3795
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 53
    Illegal EcoRI site found at 4411
    Illegal SpeI site found at 3236
    Illegal PstI site found at 1547
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 53
    Illegal EcoRI site found at 4411
    Illegal SpeI site found at 3236
    Illegal PstI site found at 1547
    Illegal AgeI site found at 2941
    Illegal AgeI site found at 3053
    Illegal AgeI site found at 5695
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 910
    Illegal BsaI site found at 1622


[edit]
Categories
Parameters
None